AJTR Copyright © 2009-All rights reserved. Published by e-Century Publishing Corporation, Madison, WI 53711
Am J Translational Res 2011;3(1):61-72

Original Article
Conditional PPARgamma knockout from cardiomyocytes of adult mice
disturbs myocardial fatty acid utilization

Jinwen Luo, Sijie Wu, Jian Liu, Yuquan Li, Huan Yang, Teayoun Kim, Olga Zhelyabovska, Guoliang Ding, Yiqun
Zhou, Yifeng Yang and Qinglin Yang

Department of Nutrition Sciences, University of Alabama at Birmingham, 1675 University Blvd, Birmingham, AL,
USA; Department of Cardio-thoracic Surgery, The Xiangya Medical College, Zhongnan University, Changsha,
China; Department of Anatamy, The Second Milatary Medical University, Shanghai, China; Department of
Pediatrics, Emory University, Atlanta, GA, USA

Received September 23, 2010; accepted September 30, 2010; October 1, 2010; Published January 1, 2011

Abstract: While the roles of PPARalpha and PPARdelta (beta) in transcriptional regulation of myocardial lipid
metabolisms are well established, an essential role of PPARgamma in regulating lipid metabolisms in the adult
heart remains unclear. In this study, we investigate whether PPARgamma is required for normal myocardial lipid
metabolism at basal condition in adult mice. We assessed the short-term cardiomyocyte-restricted PPARgamma
knockout mice with a Tamoxifen inducible Cre-LoxP mediated gene targeting strategy. The expression of
PPARgamma mRNA and protein in cardiomyocytes of adult mice was substantially reduced after short-term
induction. Transcript and protein levels of important proteins in fatty acid uptake and oxidation, such as CD36,
heart type-fatty acid binding protein (FABP), and carnitine palmitoyltransferase I (CPT-I) were reduced in the
PPARgamma deficient hearts. Myocardial fatty acid utilization and cardiac contraction was depressed in
PPARgamma deficient hearts. The PPARgamma deficient hearts exhibited modest cardiac hypertrophy
compared with controls. These results indicate that PPARgamma is a transcription factor that is required for
basal myocardial fatty acid utilization in the adult heart.  (AJTR1009002).

Key words: PPARgamma, fatty acid utilization, cardiac function, fatty acid metabolism

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Address all correspondence to:
Qinglin Yang, MD, PhD
Department of Nutrition Sciences
University of Alabama at Birmingham
1675 University Blvd
Birmingham, AL 35294-3360, USA.
E-mail:
qyang@uab.edu