Original Article Lipotoxicity in HepG2 cells triggered by free fatty acids
Hong-Rui Yao, Jun Liu, Daniel Plumeri, Yong-Bing Cao, Ting He, Ling Lin, Yu Li, Yuan-Ying Jiang, Ji Li, Jing Shang
Systems Biology Group, Drug Screening Center, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, Jiangsu Province, China; New Drug Research Center, Second Military Medical University,325 Guo He Road, Shanghai 200433, China; Department of Pharmacology and Toxicology, School of Medicine and Biomedical Sciences, University at Buffalo-SUNY, Buffalo, NY, USA.
Received April 27, 2011; Accepted May 10, 2011; Epub May 10, 2011; Published May 15, 2011
Abstract: The goal of this study was to investigate the lipid accumulation and lipotoxicity of free fatty acids (FFAs) induced in HepG2 cells. HepG2 cells were co-incubated with various concentrations of FFAs for 24h and the intracellular lipid contents were observed by Oil Red O and Nile Red staining methods. The lipotoxicity of HepG2 cells were then detected by Hoechest 33342/PI, Annexin V-FITC/PI double-staining and 3-(4,5-dimethylthiazol-2-yl)-2,5-di phenyltetrazolium bromide (MTT) experiment tests. The experiments showed a lipid accumulation and lipotoxicity by increasing FFA concentration gradients. Through cell morphological observation and quantitative analysis, FFAs have shown to increase in a dose-dependent manner compared with the control group. The data collected from hoechst 33342/PI, annexin V-FITC/PI double staining and also MTT experiments showed that cell apoptosis and necrosis significantly increased with increasing FFA concentrations. Apoptosis was not obvious in the 1 mM FFAs-treated group compared to the other two groups. In a certain concentration range, FFAs induced intracellular lipid accumulation and lipotoxicity of HepG2 cells in a dose-dependent manner. (AJTR1104004).